The article is devoted to research on the influence of the separation layer wood-concrete floor structures. Using the separation layer is justified especially in the case of reconstruction of historically valuable floor structures, such as a painted deck or beams, where water during the casting technology may cause damage, or even in the case of concrete applications with a high coefficient of water, where excessive moisture could adversely affect the mechanical properties of wood.
According to the results of the experiments we can say that in the application of concrete with a water factor approximately 0.4, it is not necessary to use concrete structure interlayer separation between concrete and formwork. Using foil separation negatively affects stress coupling elements, and thus the entire composite structure.