The second generation of the design standard, FprEN 1992-1-1:2023, allows for the use of fibre-reinforced polymer (FRP) reinforcement in structural concrete for the first time in Czech design practice. Reinforcement made of glass (GFRP) or carbon fibre (CFRP) is a promising alternative, especially in environments with high demands for corrosion resistance. The reinforcement is non-conductive and non-magnetic and is therefore suitable for areas with shielding requirements against the electromagnetic waves. Compared to steel, however, GFRP has a lower modulus of elasticity and a linear response to imposed loads up to failure, which requires a different approach to structural sizing. The new standard defines a relationship for determining the design punching shear capacity of a slab with longitudinal FRP reinforcement but does not allow the FRP to be used as shear reinforcement. This paper presents experimental results from slabs reinforced with both traditional steel and FRP reinforcement. Selected specimens also included FRP stirrups to assess their effect on punching shear capacity. The experimental results are compared with predictions based on both the first and second generation of the Eurocode.