This contribution deal with the testing of thermal resistance and derived thermal transmittance of timbered wall from spruce wood beams 300 mm thick according to the standard EN ISO 8990 by Hot Guarded Box method. Thermal conductivity tests of individual layers made from the tested wall were carried out according to the standard ISO 8302 by Guarded Hot Plate method after the test according to the standard EN ISO 8990. These thermal conductivity tests were carried out for the average thermal resistance determination of the wall from the equivalent thermal conductivity and also for the comparison with the value founded by Hot Guarded Box method. The part of the tests was also the determination of mass moisture of individual layers and then the equivalent mass moisture of the wall. The contribution contains the maximal requirement values of thermal transmittance of the exterior walls according to the national standard ČSN 73 0540-2. The contribution contains also informative table of thermal conductivities of the plates of different wood profiles founded by tests or from literature.