Currently, in the EU, the demand for new organic materials based on rapidly renewable natural sources is increasing. These materials are not only used for the new low energy and passive buildings, but also in existing buildings, specially during their renovations. Unlike those commonly used today, these natural materials have acoustic insulation properties, which make them ideal to be used on the insulation of floating floors. The paper deals with a comprehensive assessment of the insulation properties of hemp based materials, which can be used in floating floors, specially in terms of their acoustic insulation properties and also in terms of the total utility properties of these floating floors.